Karatatiwant (Karat) Singh Sidhu
Bio
Welcome to my personal portfolio. I'm Karat. 👋
I began my academic pursuits in Biotechnology at Panjab University, Chandigarh, India, earning my B.E. from the University Institute of Engineering and Technology in 2015. Continuing my education, I pursued an M.S. in Biotechnology at Texas A&M University, College Station, Texas, graduating in 2017.
During my graduate studies, I focused on analytical chemistry and biochemistry, gaining extensive experience with Triple Quadrupole Mass Spectrometers and High-Performance Liquid Chromatography Equipment. I refined my skills in a high-throughput BSL-2 lab at the Center for Translational Research in Aging and Longevity under Dr John Thaden, where we developed and quantified small molecules and metabolomics assays.
My research interests primarily center around metabolomics, proteomics, and small molecule analysis, often incorporating stable isotopes for enhanced precision, facilitated by LC/MS/MS technology..
For the past five years, I've had the privilege of contributing to the University of Vermont Mass Spectrometry Core Facility, housed within the Vermont Center for Immunobiology and Infectious Disease (VCIID). Working closely with Dr Dwight E Matthews, I utilized High-Resolution Mass Spectrometers (HRMS) and Ultra High-Performance Liquid Chromatography (UPLC) to elucidate metabolic pathways in vivo and in cell culture settings, fostering advancements in cell culture and metabolism research.
Feel free to explore my work further and please don't hesitate to reach out.
Find me on: Twitter, LinkedIn or feel free to send me an EMail.
Experience
- Lab Research Technician, University of Vermont July 2017 - December 2021
- Graduate Research Assistant, Texas A&M University Feb 2016 - May 2017
- Isotope Analysis - Intern, Texas A&M University May 2016 - August 2016
- Graduate Research Assistant - Tech Commercialization, Texas A&M University January 2016 - May 2016
- HPLC/ R&D - Intern, Ind-Swift Industries , Mohali, India June 2014 - July 2014
- Vaccine Development - Intern, Central Research Institute, Kasauli, India June 2013 - July 2013
Skills & Techniques
LC/MS
- AB SCIEX Q-Trap 5500, AB SCIEX API 4000, AB SCIEX API 2000 Waters Xevo-G2-XS QTOF, Acquity M-Class UPLC, Eksigent microLC200, Eksigent HT-Ultra, CTC-PAL
Software
- Analyst, Multi-Quant, PLGS, Progenesis QI, XCMS, TargetLynx, El-Maven, Microsoft Excel
Techniques
- Cell & Tissue Culture, Analytical Chemistry ,HPLC/UPLC, MS/MS, PCR, Electrophoresis, Sample Prep, Method Development
Code
- R , Python
Publications*
2023
Sidhu K.S. (2023), MetaboCorr: Visualizing metabolomics data using Correlation Matrices using an online application - OPENAIRE https://doi.org/10.5281/zenodo.7674084
Sidhu K.S. (2023). MetabHeatmap: A R/Shiny based app for visualizing metabolomics data through heatmaps - Zenodo https://doi.org/10.5281/zenodo.7631526
2022
Sidhu K.S. (2022), VSCode vs RStudio — Worth the Switch? - Towards Data Science . https://towardsdatascience.com/vscode-vs-rstudio-worth-the-switch-7a4415fc3275
2021
Sidhu, K. S., Amiel, E., Budd, R. C., & Matthews, D. E. (2021). Determination of cell volume as part of metabolomics experiments. American Journal of Physiology-Cell Physiology, 321(6), C947–C953. https://doi.org/10.1152/ajpcell.00613.2020
Giddings, E. L., Champagne, D. P., Wu, M.-H., Laffin, J. M., Thornton, T. M., Valenca-Pereira, F., Culp-Hill, R., Fortner, K. A., Romero, N., East, J., Cao, P., Arias-Pulido, H., Sidhu, K. S., Silverstrim, B., Kam, Y., Kelley, S., Pereira, M., Bates, S. E., Bunn, J. Y., … Rincon, M. (2021). Mitochondrial ATP fuels ABC transporter-mediated drug efflux in cancer chemoresistance. Nature Communications, 12(1). https://doi.org/10.1038/s41467-021-23071-6
2020
Abreu, S. C., Hampton, T. H., Hoffman, E., Dearborn, J., Ashare, A., Sidhu, K. S., Matthews, D. E., McKenna, D. H., Amiel, E., Barua, J., Krasnodembskaya, A., English, K., Mahon, B., Santos, C. D., Cruz, F. F., Chambers, D. C., Liu, K. D., Matthay, M. A., Cramer, R. A., … Enes, S. R. (2020). Differential effects of the cystic fibrosis lung inflammatory environment on mesenchymal stromal cells. American Journal of Physiology-Lung Cellular and Molecular Physiology, 319(6), L908–L925. https://doi.org/10.1152/ajplung.00218.2020
*Publications may not be upto date.
Awards & Honours
- Biotechnology Graduate Student Fellowship, Texas A&M University 2015 & 2016
- Biotechnology Advisory Council Award Texas A&M University, 2016
- Certificate of Entrepreneurship, Mays Business School, 2017
- Outstanding Graduate Award , Texas A&M University 2017